Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Cyclooxygenase-1 mediates neuroinflammation and neurotoxicity in a mouse model of retinitis pigmentosa

Fig. 2

COX-1 deletion reduces microgliosis in rd10 mice. ah Retina flat mounts were stained with an antibody against Iba-1, a marker for microglia. Confocal images from the dorsal retina at about 1 mm away from the optic nerve head along the dorsal-ventral axis show that microglia have a ramified morphology in P25 and P30 WT retinas (a, e, arrows). Similarly, microglia show a ramified morphology in P25 and P30 COX-1−/− mouse retinas (b, f, arrows). Microglia display an amoeboid morphology with few processes and rounded cell bodies in P25 and P30 rd10 retinas (d, h, arrowheads), whereas microglia maintain a ramified appearance in age-matched COX-1−/−/rd10 mouse retinas (c, g, arrows). Scale bar: 20 μm. il ELISA analyses of TNF-α (i; F(3, 20) = 40.19, p = 1.2 × 10−8; WT versus COX-1−/−, p = 0.52; WT versus rd10, p = 1.3 × 10−5; WT versus COX-1−/−/rd10, p = 0.198; COX-1−/− versus rd10, p = 1.1 × 10−5; COX-1−/− versus COX-1−/−/rd10, p = 0.11; rd10 versus COX-1−/−/rd10, p = 1.2 × 10−5), IL-1β (j; F(3, 20) = 15.59, p = 1.8 × 10−5; WT versus COX-1−/−, p = 0.71; WT versus rd10, p = 3.5 × 10−5; WT versus COX-1−/−/rd10, p = 0.286; COX-1−/− versus rd10, p = 1.3 × 10−5; COX-1−/− versus COX-1−/−/rd10, p = 0.37; rd10 versus COX-1−/−/rd10, p = 0.0005), and PGE2 (k; F(3, 20) = 58.85, p = 4.2 × 10−10; WT versus COX-1−/−, p = 0.71; WT versus rd10, p = 2.4 × 10−7; WT versus COX-1−/−/rd10, p = 0.20; COX-1−/− versus rd10, p = 4.8 × 10−6; COX-1−/− versus COX-1−/−/rd10, p = 0.24; rd10 versus COX-1−/−/rd10, p = 4.8 × 10−7) protein expression levels in P25 WT, rd10, and COX-1−/−/rd10 mouse retinas. l qPCR analyses of EP2 mRNA levels in sorted primary microglial cells labeled with FITC-CD11b by FACS from P25 WT, rd10, and COX-1−/−/rd10 mouse retinas (F(3, 20) = 25.62, p = 4.7 × 10−7; WT versus COX-1−/−, p = 0.56; WT versus rd10, p = 1.4 × 10−6; WT versus COX-1−/−/rd10, p = 0.087; COX-1−/− versus rd10, p = 1.2 × 10−5; COX-1−/− versus COX-1−/−/rd10, p = 0.11; rd10 versus COX-1−/−/rd10, p = 0.00033). Results are presented as means ± SDs (n = 6 animals/each group). ns not significant and ***p < 0.001

Back to article page