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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Cyclooxygenase-1 mediates neuroinflammation and neurotoxicity in a mouse model of retinitis pigmentosa

Fig. 3

COX-1 deletion preserves photoreceptor morphology in rd10 mice. ah Retinal flat mounts were stained with an antibody against red/green opsins. Retinas from the dorsal retina at about 1 mm away from the optic nerve head along the dorsal-ventral axis of WT mice show red/green-opsin-expressing cone outer segment (OS) (a, e, arrows in insets). Similarly, elongated cone OSs were shown in P25 and P30 COX-1−/− mouse retinas (b, f, arrows in insets). Cone OS and inner segment (IS) in the retina of P25 and P30 rd10 mice were revealed by the antibody showing that cone OS and IS became flattened (d, h, arrows in insets), whereas cone OS and IS remained partially intact in age-matched COX-1−/−/rd10 mice (c, g, arrows in insets). il Retinal sections from the dorsal retina at about 1 mm away from the optic nerve head along the dorsal-ventral axis of P30 C57BL/6J (i), COX-1−/− (j), COX-1−/−/rd10 (k), and rd10 (l) mice were stained with hematoxylin and eosin (H&E). ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Scale bar, 20 μm. m Plot of the average length of cone OS/IS, measured in retinal vertical sections of three groups of mice at P25 (F(3, 20) = 23.12, p = 1.0 × 10−6; WT versus COX-1−/−, p = 0.45; WT versus rd10, p = 2.3 × 10−5; WT versus COX-1−/−/rd10, p = 0.00058; COX-1−/− versus rd10, p = 4.0 × 10−5; COX-1−/− versus COX-1−/−/rd10, p = 0.0019; rd10 versus COX-1−/−/rd10, p = 0.00016), and P30 (F(3, 20) = 45.52, p = 3.3 × 10−9; WT versus COX-1−/−, p = 0.28; WT versus rd10, p = 1.3 × 10−6; WT versus COX-1−/−/rd10, p = 2.6 × 10−5; COX-1−/− versus rd10, p = 1.7 × 10−6; COX-1−/− versus COX-1−/−/rd10, p = 6.7 × 10−5; rd10 versus COX-1−/−/rd10, p = 0.00061). n Quantification of cone cell densities from whole mounted retinas of WT, rd10, and COX-1−/−/rd10 mice at P25 (F(3, 20) = 0.98, p = 0.42; WT versus COX-1−/−, p = 0.39; WT versus rd10, p = 0.066; WT versus COX-1−/−/rd10, p = 0.15; COX-1−/− versus rd10, p = 0.41; COX-1−/− versus COX-1−/−/rd10, p = 0.75; rd10 versus COX-1−/−/rd10, p = 0.33), and P30 (F(3, 20) = 27.62, p = 2.6 × 10−7; WT versus COX-1−/−, p = 0.53; WT versus rd10, p = 1.2 × 10−5; WT versus COX-1−/−/rd10, p = 0.0002; COX-1−/− versus rd10, p = 4.5 × 10−5; COX-1−/− versus COX-1−/−/rd10, p = 0.0008; rd10 versus COX-1−/−/rd10, p = 0.012). o Plot of the thickness of the outer nuclear layer (ONL), measured in numbers of photoreceptor nuclear row per column in retinal vertical sections of three groups of mice at P25 (F(3, 20) = 65.62, p = 1.6 × 10−10; WT versus COX-1−/−, p = 0.62; WT versus rd10, p = 6.7 × 10−7; WT versus COX-1−/−/rd10, p = 1.6 × 10−6; COX-1−/− versus rd10, p = 1.1 × 10−6; COX-1−/− versus COX-1−/−/rd10, p = 2.5 × 10−6; rd10 versus COX-1−/−/rd10, p = 0.014), and P30 (F(3, 20) = 55.14, p = 7.5 × 10−10; WT versus COX-1−/−, p = 0.69; WT versus rd10, p = 4.1 × 10−7; WT versus COX-1−/−/rd10, p = 5.9 × 10−6; COX-1−/− versus rd10, p = 1.8 × 10−6; COX-1−/− versus COX-1−/−/rd10, p = 2.9 × 10−5; rd10 versus COX-1−/−/rd10, p = 1.9 × 10−5). Results are presented as means ± SDs (n = 6 animals/each group). ns, not significant, *p < 0.05 and ***p < 0.001

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