Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Cyclooxygenase-1 mediates neuroinflammation and neurotoxicity in a mouse model of retinitis pigmentosa

Fig. 6

Inhibition of the prostaglandin receptor EP2 by TG6-10-1 reduces microgliosis in rd10 mice. Confocal images from the dorsal retina at about 1 mm away from the optic nerve head along the dorsal-ventral axis show that microglia display a ramified morphology in P25 WT (a, arrows). Microglia maintained a ramified appearance in TG6-10-1-treated rd10 retinas (b, arrows), whereas microglia show an amoeboid morphology in DMSO-treated rd10 controls (c, arrowheads). Scale bar: 20 μm. d qPCR analyses of EP2 mRNA levels in retinal microglial cells sorted by FACS from P25 CX3CR1+/GFP and rd10/CX3CR1+/GFP mice treated with TG6-10-1 or DMSO (F(2, 15) = 32.61, p = 3.5 × 10−6; WT versus DMSO-treated rd10, p = 6.4 × 10−6; WT versus TG6-10-1 treated rd10, p = 0.027; DMSO-treated rd10 versus TG6-10-1 treated rd10, p = 0.00016). e ELISA analyses of TNF-α expression levels in the retina of three groups (F (2, 15) = 33.19, p = 3.1 × 10−6; WT versus DMSO- treated rd10, p = 4.7 × 10−6; WT versus TG6-10-1 treated rd10, p = 0.011; DMSO-treated rd10 versus TG6-10-1 treated rd10, p = 0.00036). f ELISA analyses of IL-1β expression levels in the retina of three groups (F(2, 15) = 210.37, p = 1.1 × 10−11; WT versus DMSO- treated rd10, p = 8.9 × 10−10; WT versus TG6-10-1 treated rd10, p = 0.015; DMSO-treated rd10 versus TG6-10-1 treated rd10, p = 2.5 × 10-8). g ELISA analyses of COX-1 expression levels in the retina of three groups (F(2, 15) = 56.53, p = 1.1 × 10−7; WT versus DMSO-treated rd10, p = 1.9 × 10−7; WT versus TG6-10-1 treated rd10, p = 0.0071; DMSO-treated rd10 versus TG6-10-1 treated rd10, p = 3.6 × 10−5). h ELISA analyses of PGE2 expression levels in the retina of three groups (F(2, 15) = 96.95, p = 2.6 × 10−9; WT versus DMSO-treated rd10, p = 3.1 × 10−7; WT versus TG6-10-1 treated rd10, p = 0.058; DMSO-treated rd10 versus TG6-10-1 treated rd10, p = 5.5 × 10−7). Results are presented as means ± SDs (n = 6 animals/each group). ns not significant, *p < 0.05, **p < 0.01, and ***p < 0.001

Back to article page