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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Role of macrophage-mediated Toll-like receptor 4–interleukin-1R signaling in ectopic tongue pain associated with tooth pulp inflammation

Fig. 5

Effects of Hsp70 or IL-1β injection in the trigeminal ganglion. a Time course of the experimental procedure for the groups administered vehicle or Hsp70 into the trigeminal ganglion. b Head withdrawal threshold to heat or mechanical stimulation of the ipsilateral tongue following administration of Hsp70 into the trigeminal ganglion. Two-way repeated-measures ANOVA followed by Tukey’s post hoc tests were used to compare head withdrawal thresholds between groups. c Fluorogold-labeled IL-1RI-IR cells following Hsp70 injection into the trigeminal ganglion. The arrowheads indicate fluorogold-labeled IL-1RI-IR cells. Scale bar, 100 μm. d The number of each IR cell type in the trigeminal ganglion of the third branch region was calculated using the following formula: fluorogold-labeled IL-1RI-IR cells/fluorogold-labeled cells × 100%. The Student’s t test was employed for the comparison of percentages of positive cell between groups. e Time course of the experimental procedure for the groups administered vehicle or Hsp70 into the trigeminal ganglion. f Head withdrawal threshold to heat or mechanical stimulation of the ipsilateral tongue following IL-1β injection into the trigeminal ganglion. Two-way repeated-measures ANOVA followed by Tukey–Kramer post hoc tests were used to compare head withdrawal thresholds between groups. g, h Fluorogold-labeled TRPV1-IR cells following IL-1β injection into the trigeminal ganglion. The arrowheads indicate fluorogold-labeled TRPV1-IR cells. Scale bar, 100 μm. The number of each IR cell type in the trigeminal ganglion of the third branch region was calculated by using the following formula: fluorogold-labeled TRPV1-IR cells/fluorogold-labeled cells × 100%. The Student’s t test was employed for the comparison of percentages of positive cells between groups. Data are expressed as the mean ± SEM

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