Fig. 2

Sex-biased depletion of CD11b+ cells in the CNS of CLN1 mice upon treatment with PLX3397. a, b Representative fluorescent microscopic images of immunohistochemically labeled CD11b⁺ microglia/macrophages of male and female PLX3397-treated Ppt1^−/− mice in longitudinal optic nerve and S1Bf cortex sections, respectively. Scale bar: 50 μm. c, d Quantification of CD11b⁺ cells showed a significant sex bias, with a higher percentage of depletion in male Ppt1^−/− mice treated with PLX3397. n = 5 male and 5 female mice per group. One-way ANOVA and Tukey’s post hoc tests. **^{, ##}P < 0.01; ***^{, ###}P < 0.001. # significant difference to untreated Ppt1^−/− mice of the same sex. Datasets also shown in Fig. 1 are presented separately for sex