Fig. 6
From: Sex- and region-biased depletion of microglia/macrophages attenuates CLN1 disease in mice
Depletion of microglia/macrophages reduces retinal thinning and ganglion cell loss and attenuates brain atrophy in CLN1 mice. a Longitudinal OCT analysis of the innermost retinal composite layer thickness in Ppt1+/+, Ppt1−/−, and PLX3397-treated Ppt1−/− mice from 4 to 6 months of age. Retinal thinning was significantly delayed and less pronounced in PLX3397-treated Ppt1−/− mice. b Representative fluorescent microscopic images of immunohistochemically labeled RBPMS+ retinal ganglion cells in flat mounts of 6-month-old Ppt1+/+, Ppt1−/− mice and PLX3397-treated Ppt1−/− mice. Scale bar: 20 μm. c Quantification of RBPMS+ retinal ganglion cells showed a significant reduction of neuron loss in PLX3397-treated Ppt1−/− mice. d Analysis of total brain weight showed that depletion of microglia/macrophages resulted in attenuation of brain atrophy in PLX3397-treated CLN1 mice. n = 5 male and 5 female mice per group. One-way ANOVA and Tukey’s post hoc tests. *P < 0.05; **P < 0.01; ***P < 0.001