Fig. 1

Effects of I3C on pro-inflammatory and anti-oxidant mRNA and protein levels. a-h BV-2 microglia cells were treated with 50 μM I3C or DMSO as a vehicle for 4 h followed by 50 ng/ml LPS for 4 h. After 8 h, mRNA expression levels of i-NOS a, IL-1ß b, NLRP3 c, IL-6 d, CCL2 e, NQO1 f, HMOX1 g, and CAT1 h were analyzed by real-time PCR. i Western blots show i-NOS, IL-1ß, COX2, NQO1, HMOX1, and ß-ACTIN protein levels as a loading control. Mean relative protein levels are shown for i-NOS j, IL-1ß k, and HMOX1 l. Data show mean ± SEM out of three independent experiments for mRNA expression levels (n = 3/group, measured in triplicates). For Western blot, three independent experiments were performed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001