Fig. 5

Akt/CREB/BDNF might be the possible downstream signaling pathway of GR- and MR-mediated effects on apoptosis in the PVN after TBI. a Representative western blot images showing BDNF, p-Akt, Akt, P-CREB, and CREB expression. b–d Quantification of BDNF, p-Akt/Akt, and p-CREB/CREB levels. The relative band density was measured with Image J (1.49 V) and normalized to that of β-actin, and the percent expression compared to that of sham controls was calculated for each sample. e Representative images of BDNF (green, white arrow) and CRH (red, yellow arrow) immunofluorescence in the PVN. ^*P < 0.05 compared to the sham group, and ^#P < 0.05 compared to the CCI control group. The data are presented as the means ± SD of 6 animals per group