Fig. 2
From: Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia
Irradiation induces microglial senescence in vivo. a Schematic diagram of the isolation of primary microglia from 6-week-old C57BL/6 J male mice after IR at 20 Gy using a linac. b The expression level of microglial markers, including ITGAM, CX3CR1, and CD68, in primary microglia (mean ± SEM, ***P < 0.001). c The activity of β-galactosidase of irradiated microglia isolated from C57BL/6 J male mice at different day post-irradiation (dpi) is shown as the mean ± SEM; **P < 0.01, versus the control group. d Adult male C57Bl6/J mice were exposed to a single dose of IR (10 Gy) by linac. Representative sections from cortex immunostained with anti-Iba-1 (red) and anti-P16INK4A (green) antibodies at different dpi are shown (scale bar, 40 μm). e The ratio of P16INK4A positive microglia in the cortex is shown as the mean ± SEM; ***P < 0.001, versus the control group