Fig. 6

The reciprocal relationship between DNA damage and the RILs. a The protein levels of γh2A.X in BV2 cells a 1 h after exposure to 10 Gy using a linac or X-ray irradiation, as revealed using western blotting. b Histogram of the quantification of the immunoreactive protein bands on the western blot (mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001). c BV2 cells were exposed to 10 Gy using a linac or X-ray irradiation, and the relative expression of ten randomly selected RILs from the RNA-seq data (seven upregulated and three downregulated) at different time points were measured using qRT-PCR. The relative expression levels were normalized using the Z-score and presented in a heatmap. Hash (#) represents the only RIL in X-ray irradiation group that shared the same expression trend with RNA-seq. d The protein levels of γh2A.X in cells treated with shRNA targeting RILs or NC at 1 h after exposure to 10 Gy using a linac in BV2, as revealed using western blotting. e Histogram of the quantification of the immunoreactive protein bands on the western blot (mean ± SEM, ns, not significant, *P < 0.05, **P < 0.01)