Fig. 8
From: Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia
Blockade of RILs alleviated neuronal damage after radiation. a Neuron HT22 cells were co-cultured with irradiated BV2 cells with/without ENSMUST00000190863 or ENSMUST00000130679 knockdown for 24 h. Apoptotic HT22 cells were identified using TUNEL staining. Representative micrographs are shown. Scale bar = 100 μm. b Apoptotic HT22 cells were quantified using flow cytometry with Annexin V-APC/7-AAD staining. c The ratio of apoptotic cells is shown (mean ± SEM, **P < 0.01, ***P < 0.001). d Schematic diagram of the reciprocal relationship between the senescence phenotype and RILs in radiation-treated microglia