Fig. 2

Transcriptional profiling of mIFNα exposed microglia. a Volcano plot showing highlights of differentially expressed (DE) genes from bulk RNA sequencing of sorted cortical microglia. Interferon-stimulated genes were identified based on querying the Interferome 2.0 database [43] with the DE gene list. q, Benjamini-Hochberg adjusted p value. Dotted lines demarcate q = 0.01 and fold change = 2.5. b A selection of differential gene ontology pathways identified using gProfiler2 [31] enriched in mIFNα exposed microglia. c Validation of several interferon-stimulated genes (ISGs) by ddPCR on sorted cortical microglia from an independently treated cohort of mice. N = 6 for all data shown. **p < 0.01; ***p < 0.001; Student’s t test