Fig. 3
BMAA induces impairment of OCR in primary cortical neurons. OCR representative curve and respective histograms of Basal O2 consumption, maximal O2 consumption, coupling efficiency, ATP production, and proton leak were evaluated in primary mouse cortical neurons pre-treated with 1 μM CCCP, 3 mM BMAA, and 1 μg/μl LPS for 48 h, in the presence of 1 μM oligomycin, 2 μM FCCP, and 2 μM antimycin 2 μM plus rotenone. Incubations were performed in quadruplicate and data are the mean values of such experiments. Data represent the mean ± SEM derived from five independent experiments and are expressed relative to untreated cells. The statistical significance was determined using one-way ANOVA followed by Dunnett’s test *p < 0.05, **p < 0.01, and ***p < 0.001 versus untreated neurons. N.D., negligible OCR rates