Fig. 3

Cerulenin blocks LPS-induced secretion of neurotoxins by BV2 cells. a BV2 cells were pretreated for 1 h with vehicle (0.01% DMSO), 10 μM cerulenin, or 5 μM BAY11-7082, followed by treatment for 24 h with 0 (CTL:1x PBS) or 100 ng/ml LPS to obtain conditioned media. HT22 cells grown in regular medium were switched to the conditioned media. After 24 h, cell viability was measured using MTT assay. b HT22 cells were cultured in various microglia-conditioned media (MCM) shown with or without addition of 10 μM cerulenin as indicated. Cell viability was measured as above. V, vehicle, C, cerulenin. c HT22 cells grown on chamber slides were treated as in a: pretreatment with vehicle or cerulenin followed by treatment with CTL or LPS. Fixed cells were analyzed by TUNEL assay. Nuclei were visualized with DAPI. Percent of TUNEL positive cells are shown on the right. Two-way ANOVA with Tukey’s post hoc test was used for multiple comparisons. Bar = 200 μm. Different letters indicate statistical differences at P < 0.05. Mean + SD, n = 3-4. *P < 0.05, compared to vehicle in c. #P < 0.05 compared to CTL in c