Fig. 4

Cerulenin inhibits AP-1 transcriptional activity by disrupting its interaction with TonEBP. a BV2 cells were transfected with an NF-κB luciferase reporter. Luciferase activity was measured 3 h after treatment with 100 ng/ml LPS or vehicle (CTL:1x PBS). Where indicated, some cells were pretreated for 1 h with 10 μM cerulenin before treatment with LPS. Mean + SD, n = 3. #P < 0.05, compared to LPS. *P < 0.05 compared to CTL. b BV2 cells were transfected with an AP-1 luciferase reporter. Luciferase activity was measured 3 h after treatment with 100 ng/ml LPS or vehicle (CTL:1x PBS). Where indicated, some cells were pretreated for 1 h with 10 μM cerulenin before treatment with LPS. Mean + SD, n = 4. #P < 0.05, compared to LPS. *P < 0.05 compared to CTL. c MEF cells were pretreated with cerulenin (C) or vehicle (V) followed by treatment with LPS as above. Cell lysates were immunoprecipitated using c-jun antibodies and then immunoblotted for TonEBP and c-jun as shown in left. Right panel shows band intensity for TonEBP corrected by intensity of corresponding c-jun band. **P < 0.05 compared to vehicle. #P < 0.05 compared to CTL