Fig. 5

Myeloid TonEBP is required for LPS-induced microglia activation. LPS (3 μg per animal) or vehicle (CTL:1x PBS) was injected into the left ventricle of male TonEBPfl/fl,LysM-cre mice (solid bars) and their TonEBPfl/fl littermates (open bars). Brains were analyzed 3 h later. a Brains were perfusion-fixed and immunostained for iba-1. Images from cortex (CTX) and hippocampus (HIP) (top) were analyzed for iba-1 positive areas and iba-1 positive cell numbers (bottom). Bar = 70 μm. b RNA was isolated from CTX and HIP, and analyzed by RT-qPCR for TNF-α and IL-1β. Mean + SE, n = 3–6. #P < 0.05, compared to CTL. *P < 0.05 compared to TonEBP fl/fl