Skip to main content

Table 1 Primers and probes used in the present study

From: Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations

Gene name

Primers 5'-3'

Probe number

GenBank accession number

Interleukin-6

(il6)

F gatggatgctaccaaactggat

6

NM_031168.1

R ccaggtagctatggtactccaga

  

Interleukin-1 beta

(il1b)

F gagaatgacctgttctttgaagttga

–

NM_008361.3

R agatttgaagctggatgctctca

  

P accccaaaagatgaagggctgcttcc

 

Tumor necrosis factor-alpha

(tnfa)

F tgtctactgaacttcggggtga

–

NM_013693.2

R gaagatgatctgagtgtgagggtct

  

P tccccaaagggatgagaagttccca

  

Brain derived neurotrophic factor

(bdnf)

F cacttttgagcacgtcatcg

42

*

R tccttatggttttcttcgttgg

  

Nerve growth factor transcript variant A

(ngf)

F gtgcctcaagccagtgaaa

10

NM_013609.3

R gaccacaggccaaaactcc

  

Neurotrophin 3, transcript variant 1

(nt3)

F cgacgtccctggaaatagtc

29

NM_001164034.1

R tggacatcaccttgttcacc

  

Neurotrophic tyrosine kinase, receptor, type 1

(trka)

F tgtccaagtcagcgtctcc

20

NM_001033124.1

R aaggggatgcaccaatgat

  

Neurotrophic tyrosine kinase, receptor, type 2, transcript variant 1

(trkb)

F tgcccagagcaggataagat

76

NM_001025074.2

R aaagtccttgcgtgcattgt

  

Glyceraldehyde 3-phosphate dehydrogenase

(gapdh)

F ggtgtcttcaccaccatgga

–

NM_008084.2

R cagaaggggcggagatgat

  

P aaggccggggcccacttgaa

  
  1. Listed probe numbers indicate the product number of the Universal ProbeLibrary Set, Human and Extension Set sold by Roche Applied Science
  2. *The primer sets of BDNF were designed in the same manner as previously described (Tsukahara et al. 2019)
  3. Primers and probes of IL-1β, TNF-α and GAPDH were designed and synthesized by Bioreseach Technologies Japan (Tokyo, Japan)
Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094