Fig. 3

MRS5980 attenuates the activation of NFκB and MAPKs pathways following CCI TBI. When compared to sham mice, the levels of cytoplasmic IκBα were decreased (a) and the levels of nuclear NFκB p65 (b) and phosphorylated p38 (c) and ERK1/2 (d) increased in brain tissue from mice 24 h after CCI TBI that were treated with vehicle. These events were attenuated in mice with CCI TBI and treated with MRS5980 (1 mg/kg; i.p.; a–d). Uncropped blot images are shown in the supplementary information (Figures S1, S2, S3 and S4). Data are mean ± SEM for 5 mice/group and analyzed by two-tailed, one-way ANOVA with Dunnett’s comparisons [a F(2, 12) = 1098, p = 2.57 × 10⁻¹⁴, η² = 0.995; b F(2, 12) = 180.0, p = 1.13 × 10⁻⁹, η² = 0.968; c F(2, 12) = 325.5, p = 3.52 × 10⁻¹¹; η² = 0.992, d F(2, 12) = 325.5, p = 3.52 × 10⁻¹¹, η² = 0.992]. *p < 0.05 vs. sham and †p < 0.05 vs. TBI + Veh