Fig. 4
MRS5980 attenuates NLRP3-inflammasome activation following controlled cortical impact TBI. When compared to sham mice, the expression of a NLRP3 and b cleaved caspase 1 increased in the brain of mice 24 h after controlled cortical impact and vehicle treatment. MRS5980 (1 mg/kg; i.p.) attenuated these changes. The blot in Fig. 3a was probed for NLRP3 and caspase 1 to generate images in a and b and share the same GAPDH image and data. Uncropped blot images are shown in the supplementary information (Figure S5). Data are mean ± SEM for 5 mice/group and analyzed by two-tailed, one-way ANOVA with Dunnett’s comparisons [a F(2, 12) = 430.6, p = 6.73 × 10−12, η2 = 0.986; b F(2, 12) = 79.45, p = 1.20 × 10−7, η2 = 0.930]. *p < 0.05 vs. sham and †p < 0.05 vs. TBI + Veh