Fig. 5

MRS5980 attenuates CD4⁺ and CD8⁺ T cell infiltration following controlled cortical impact TBI. When compared to the number of CD4⁺ (a; green) and CD8⁺ (e; green) T cells in the brain tissue sham mice, the number of CD4⁺ (b) and CD8⁺ (f) T cells increased in the brain tissue of mice measured 24 h after CCI and treatment with vehicle. MRS5980 (1 mg/kg; i.p.) attenuated the increase in CD4⁺ (c) and CD8⁺ (g) T cells in mice with TBI. Quantitation of CD4⁺ (d) and CD8⁺ (h) T cell infiltration measured from three brain slices for each mouse. Yellow arrows = co-localization between CD4 or CD8 (green) and DAPI (blue). All images were digitalized at a resolution of 8 bits into an array of 2048 × 2048 pixels. Bar = 20 μm. Data are mean ± SEM for 10 mice/group and analyzed by two-tailed, one-way ANOVA with Bonferroni comparisons [d: F(2, 27) = 108.0, p = 1.31 × 10⁻¹³, η² = 0.889 and h F(2, 15) = 53.14, p = 1.56 × 10⁻⁷, η² = 0.876]. *p < 0.05 vs. sham and †p < 0.05 vs. TBI + Veh