Fig. 6
MRS5980 prevents cognitive impairment by 4 weeks after closed head weight-drop TBI. a When compared to sham (n = 9), there were no differences in the ability of mice 1 week after TBI and vehicle treatment (n = 10) to discriminate between conditioned and novel objects or their placement. MRS5890 (1 mg/kg; i.p.; n = 10) had no effects on the outcome of NOPRT trials [F(2, 26) = 1.092, p = 0.351, η2 = 0.077]. Animals from the sham (n = 2), TBI + Veh (n = 1) and TBI + MRS5980 (n = 1) groups were excluded because of failure to explore both objects. b In contrast, mice with TBI and treated with vehicle (n = 9) exhibited increased impairment in recognizing the original object from novel objects or their placement compared to sham (n = 9) when tested 4 weeks after TBI. MRS5980 (1 mg/kg; i.p.; n = 9) attenuated this impairment in mice with TBI [F(2, 26) = 8.976, p = 0.00123, η2 = 0.428]. Additional animals from the TBI + Veh (n = 1) and TBI + MRS5980 (n = 1) were excluded because of failure to explore both objects. c When compared to sham mice (n = 11), the number of trials need to achieve 5 avoidances in 6 consecutive trials in the T maze increased in mice 4–5 weeks after TBI (n = 11). Mice with TBI and treated with MRS5980 (1 mg/kg; i.p.; n = 10) required fewer T maze trials to achieve learning and memory tasks than untreated mice with TBI [H(2) = 13.58; p = 0.00113]. One animal from the TBI + MRS5980 was excluded because it would not perform the task Data are mean ± SD for n mice/group and analyzed by a, b two-tailed, one-way ANOVA with Bonferroni comparisons or c Kruskal-Wallis with Dunn’s comparisons. *p < 0.05 vs. sham and †p < 0.05 vs. TBI + Veh