Fig. 7
Closed head weight-drop TBI and MRS5980 treatment do not alter mouse activity or cause anxiety. a, b Elevated plus maze test: when tested 4 weeks after closed head weight drop TBI, there were no differences in the number of entries into a or the time spent b in the open arms of the elevated plus maze between sham mice (n = 11), mice with TBI (n = 11) or mice with TBI that were treated with MRS5890 (1 mg/kg; i.p.; n = 11). c, d Open field test: When tested 4 0weeks after closed head weight drop TBI, there was a small but significant increase in time spent in the center of the open area in mice with TBI and treated with vehicle (n = 10) compared to sham group (c), but not difference in total distance travels (d). Mice with TBI that were treated with MRS5890 (1 mg/kg; i.p.; n = 11) did not exhibit any differences in time spent in the center (c) or distances traveled (d) in the open field test compared to the sham group or the mice with TBI treated with vehicle. One animal was excluded from the TBI + Veh group for not moving during testing. Data are mean ± SEM for n mice/group and analyzed by a, b Kruskal-Wallis with Dunn’s comparisons or (c, d) two-tailed, one-way ANOVA with Bonferroni comparisons. [a H(2) = 0.0368; p = 0.982; b H(2) = 0.753; p = 0.686; c F(2, 29) = 5.163, p = 0.0121, η2 = 0.262; d F(2, 29) = 1.492, p = 0.913, η2 = 0.0933]. *p < 0.05 vs. sham