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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Ocular macrophage origin and heterogeneity during steady state and experimental choroidal neovascularization

Fig. 1

Laser CNV model and flow cytometry gating strategy for mononuclear phagocytes. IBA1 (a), ICAM-2 (b), and merged (c) images of a flat-mounted choroidal lesion on day 3. d Schematic of experimental design. Red arrow indicates that the representative plots in e, and jm are from day 3 after laser injury. e Initial gating strategy demonstrating identification of singlet cells (left), delineation of live cells with removal of debris and count beads (middle left), gating of CD45+ cells (middle right), and selection of CD11b+, Lin- cells (Lin = CD4, CD8, B220, NK1.1, SiglecF, Ly6G). fm Gating strategy for identification of monocytes, dendritic cells (DCs), microglia, MHCII, MHCII+CD11c, and MHCII+CD11c+ macrophages from unlasered (fi) and laser treated (jm) mice. f, j Separation of CD45High from CD45Dim cells. h, l Delineation of microglia as CD45DimCD64+MHCIILow cells. g, k CD45HighCD64+MHCII macrophages (MHCII Macs) are increased with laser. i, m Increased CD45HighCD64+MHCII+CD11c (CD11c Macs) and CD45HighCD64+MHCII+CD11c+ macrophages (CD11c+ Macs) with laser treatment

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