Fig. 2

Reduced MHCII- macrophage numbers in Ccr2^−/− mice after laser injury. Representative ICAM2 immunofluorescence imaging (a) and quantitative analysis of CNV area for female (b) and male (c) wildtype (WT) vs. Ccr2^−/− mice. Microglia numbers were not changed by laser or genotype in female (d) or male (e) mice. f Representative flow cytometry pseudocolor plots from wildtype and Ccr2^−/− female mice from day 0, day 3, day 5, and day 7. MHCII⁻ macrophage numbers peaked in WT mice on day 3 and were blunted in Ccr2^−/− female (g) and male (h) mice. *p < 0.05; **p < 0.01; ***p < 0.001. Colored asterisk = significant difference from day 0 within genotype (blue = WT, red = Ccr2^−/− mice). Black asterisk = significant difference between genotypes on day 3 or day 7. N = 8–11 per group in both male and female mice. CNV was compared using the Mann-Whitney test (b, c). Macrophage numbers were compared using the Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison (d, e, g, h)