Fig. 3

Ablated MHCII⁺CD11c⁻ and decreased MHCII⁺CD11c⁺ macrophage numbers in Ccr2^−/− mice in response to laser injury. a Representative flow cytometry pseudocolor plots from wildtype and Ccr2^−/− female mice from day 0, day 3, day 5, and day 7. MHCII⁺CD11c⁻ macrophage numbers peaked in WT mice on day 3 and were completely unchanged in Ccr2^−/− female (b) and male (c) mice. MHCII⁺CD11c⁺ macrophage numbers peaked in WT mice on day 3 and were blunted in Ccr2^−/− female (d) and male (e) mice. *p < 0.05; **p < 0.01; ***p < 0.001. Colored asterisk = significant difference from day 0 within genotype (blue = WT, red = Ccr2^−/− mice). Black asterisk = significant difference between genotypes on day 3, day 5, or day 7. N = 8–11 per group in both male and female mice. Macrophage numbers were compared using the Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison