Fig. 5

Fate mapping of steady state tissue resident macrophages. a–c Number of macrophages in each tissue at steady state. d, e Percentages of steady state macrophages by subtype (d) or by tissue (e). f Representative contour plots of GFP⁺ vs. GFP⁻ macrophage subsets. g Percent GFP⁺ microglia were unchanged from week 1 to week 8. h Percent GFP⁺MHCII⁻ macrophages were equivalent from week 1 to week 8. i Percent GFP⁺MHCII⁺CD11c⁻ macrophages were decreased at week 4 and week 8. j Percent GFP⁺MHCII⁺CD11c⁺ macrophages were decreased at week 4 and week 8. k Percent GFP⁺ macrophage subsets at week 8 were significantly different. *p < 0.05; **p < 0.01; ***p < 0.001. N = 4–8 per group. Macrophage numbers were compared using the Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison