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Table 1 Antibodies used in this study

From: Ocular macrophage origin and heterogeneity during steady state and experimental choroidal neovascularization

Antibody Fluorophore Clone Usage Manufacturer
Rat anti-mouse CD16/CD32 N/A 2.4G2 Fc block BD Biosciences
Mouse anti-mouse CD64 PE X54-5/7.1 Eye BioLegend
Hamster anti-mouse CD11c BV 421 HL3 Eyea BD Biosciences
Rat anti-mouse Ly6G PE-CF594 1A8 Eye BD Biosciences
Mouse anti-mouse NK1.1 PE-CF594 PK136 Eye BD Biosciences
Rat anti-mouse Siglec F PE-CF594 E50-2440 Eye BD Biosciences
Rat anti-mouse B220 PE-CF594 RA3-6B2 Eye BD Biosciences
Rat anti-mouse CD8 PE-CF594 53-6.7 Eye and blood BD Biosciences
Rat anti-mouse CD4 PE-CF594 RM4-5 Eye and blooda BD Biosciences
Rat anti-mouse MHC II AlexaFluor 700 M5/114.15.2 Eye BioLegend
Rat anti-mouse CD11b APC-Cy7 M1/70 Eyea BD Biosciences
Rat anti-mouse CD45 PE-Cy7 30-F11 Eye and blooda BD Biosciences
Rat anti-mouse Ly6G PerCP-Cy5.5 1A8 Blooda BD Biosciences
Rat anti-mouse CD11b eFluor 450 M1/70 Blooda Invitrogen
Rat anti-mouse CD19 APC 1D3 Blooda BD Biosciences
Mouse anti-mouse NK1.1 AlexaFluor 700 PK136 Blood BD Biosciences
Rat anti-mouse CD115 PE AFS98 Blood Invitrogen
Rat anti-mouse CD19 PE 1D3 Compensation BD Biosciences
Rat anti-mouse CD19 AlexaFluor 700 1D3 Compensation BD Biosciences
Fixable viability dye eFluor 506 N/A Eyea Invitrogen
Rat anti-mouse CD102 (ICAM2) N/A 3C4(mIC2/4) Immunofluorescence BD Biosciences
Rabbit anti-mouse IBA1 N/A 019-19741 Immunofluorescence Wako
Donkey anti-rat (H+L) AlexaFluor 488 N/A Immunofluorescence Invitrogen
Donkey anti-rabbit (H+L) AlexaFluor 647 N/A Immunofluorescence Invitrogen
  1. List of antibodies, fluorophores, manufacturers, and clones. aAntibody also used for compensation setup