Fig. 3

Microglial expression of C3ar1 in healthy and inflammatory states. a Average data from single cell RNA sequencing of healthy brain tissue performed by the Betsholtz laboratory [50, 51] show little to no expression C3ar1 in cell types other than microglia. b Average data from single cell RNA sequencing of brain tissue performed by the Barres laboratory [52] show little to no expression C3ar1 in cell types other than microglia. c Single-cell RNA sequencing data are shown from the laboratories of Amit^† [53] and Stevens^‡ [54]. These studies defined sub-types or clusters of microglia based on differences in gene expression. The relative expression of C3ar1 in microglia was higher in microglia sub-types associated with macrophage-like activity or inflammation, which are shown here. d Expression of C3ar1 in RNA sequencing data from pooled cells of the indicated cell type sorted from retina or optic nerve tissue from 9 month old DBA/2J mice performed by the John laboratory [55, 56]. Astrocytes (AC), disease-associated microglia for 5xFAD mice (DAM), endothelial cells (EC), endothelial-related cells (E), fibroblast-like (FB), microglia (MG), monocytes (Mono), neurons (N), oligodendrocytes (OL), OL progenitors (OPC), pericytes (PC), smooth muscle cells (SMC), and microglia subtype clusters from Stevens: embryonic microglia (C1), postnatal microglia (C4), Ms4a7-positive microglia (C6), Ccl4-positive microglia (C8), sub-type in aging mice (AC2)