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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Complement peptide C3a receptor 1 promotes optic nerve degeneration in DBA/2J mice

Fig. 4

Isolation and RNA sequencing of C3ar1−/− and C3ar1+/+ microglia from primary culture. a Cells from primary mixed glial cultures were sorted using FACS. A population of microglia was selected for sequencing from live cells (P1) based on high expression of IBA1 and low expression of GFAP (P2). Conversely, a population of astrocytes with high GFAP expression is indicated by P3. b The sequenced cells were enriched in microglia based on a high level of expression of microglia genes Cx3cr1 and Tmem119 and a low level of astrocyte genes Aldh1l1 and Gfap. c Changes in gene expression between C3ar1−/− and C3ar1+/+ microglia visualized by MA plot. Points that represent the 408 differentially expressed genes (FDR < 0.005) are colored red

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