Fig. 2

Protection of retinal ganglion cells. a RGCs were stained with the antibody RBPMS (green). Apoptotic (red, cl. caspase 3, arrows) RGCs (green, Brn3a) and cell nuclei (blue) were also labeled with specific antibodies on retinal cross-sections. b Intravitreally injected S100B triggered an RGC loss compared to the PBS and naïve group. Some RBPMS⁺ RGCs were protected via minocycline in comparison to the S100B group, but these differences were not significant. c A higher apoptotic rate of RGCs was noted in the S100B group compared to both controls. In contrast, minocycline treatment reduced the apoptotic rate in a dose-dependent manner. Abbreviations: GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer , OPL: outer plexiform layer, ONL: outer nuclear layer. Scale bar: 20 μm, n = 6/group,**p < 0.01 vs. naïve group, ^#p < 0.01, ^##p < 0.05 vs. PBS group, ^¥¥p < 0.01 vs. S100B group