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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model

Fig. 4

Diminished microglia activity in the retina. a Iba1 antibody (red) was used to label all phagocytes and combined with Tmem119 antibody (green) to distinguish between microglia (Tmem119+ and Iba1+) and recruited phagocytes (Tmem119 and Iba1+). Active microglia/macrophages were identified by the co-localization of ED1 (green; arrows) and Iba1. b The number of Iba1+ cells was increased in the S100B group compared to both controls. Minocycline treatment reduced the S100B effect significantly. c The number of microglia (Tmem119+ and Iba1+) was upregulated in the S100B group compared to the naïve and PBS group. Both minocycline treatments significantly reduced this upregulation. d The number of phagocytes (Tmem119 and Iba1+) was similar in all groups. e Also, higher active microglia/macrophage counts were noted in the S100B group in contrast to both control groups. ED1+ and Iba1+ cell numbers in the minocycline groups were reduced. Abbreviations: GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer. Scale bar: 20 μm, n = 6/group, ***p < 0.001 vs. naïve group, ##p < 0.01, ###p < 0.001 vs. PBS group, ¥¥p < 0.01, ¥¥¥p < 0.001 vs. S100B group

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