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Table 3 RBPMS, cleaved caspase 3, Iba1, Tmem119, and ED1 cell counts (cells/mm in the retina and mm2 in the optic nerve), PKM, GFAP, and S100B area evaluations (%), as well as SMI-32 and H&E scores for immunofluorescence stains in naïve, PBS, S100B, 13.5 mg/kg mino, and 25 mg/kg mino animals. Values are displayed as mean ± SEM. p values < 0.05 are shown in bold

From: Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model

  Cell type Mean ± SEM P-values
   Naïve PBS S100B 13.5 mg/kg mino 25 mg/kg mino Naïve vs. PBS Naïve vs. S100B Naïve vs. 13.5 mg/kg mino Naïve vs. 25 mg/kg mino PBS vs. S100B PBS vs. 13.5 mg/kg mino PBS vs. 25 mg/kg mino S100B vs. 13.5 mg/kg mino S100B vs. 25 mg/kg mino 13.5 vs. 25 mg/kg mino
Retina RBPMS+ 276.8 ± 13.6 266.3 ± 19.0 161.1 ± 22.9 230.3 ± 28.5 236.0 ± 22.0 1.00 0.008 0.56 0.68 0.02 0.77 0.86 0.20 0.14 1.00
Cl. casp. 3+ + Brn3a+ 2.6 ± 0.5 3.5 ± 1.7 9.5 ± 1.0 3.3 ± 0.8 6.2 ± 1.4 1.00 0.002 1.00 0.20 0.008 1.00 0.45 0.006 0.27 0.40
Iba1+ 10.1 ± 1.0 11.7 ± 0.5 19.0 ± 0.8 11.1 ± 0.8 11.5 ± 1.0 0.66 < 0.001 0.90 0.74 < 0.001 1.00 1.00 < 0.001 < 0.001 1.00
Tmem119 + Iba1+ 9.7 ± 0.9 11.3 ± 0.4 18.6 ± 0.7 10.9 ± 0.7 10.9 ± 0.9 0.57 < 0.001 0.77 0.78 < 0.001 1.00 1.00 < 0.001 < 0.001 1.00
Tmem119- + Iba1+ 3.0 ± 1.5 3.0 ± 1.5 3.0 ± 1.5 1.5 ± 0.9 1.5 ± 0.9 1.00 1.00 0.92 0.92 1.00 0.92 0.92 0.92 0.92 1.00
ED1+ + Iba1+ 1.3 ± 0.4 2.4 ± 0.9 5.2 ± 1.2 2.5 ± 1.4 2.4 ± 1.3 0.48 < 0.001 0.40 0.48 0.002 1.00 1.00 0.002 0.002 1.00
PKM+ 14.8 ± 4.8 14.9 ± 5.3 7.5 ± 1.9 10.1 ± 3.9 9.8 ± 3.6 1.00 0.03 0.29 0.24 0.03 0.26 0.22 0.81 0.86 1.00
GFAP+ 3.1 ± 0.4 3.7 ± 0.4 3.1 ± 0.4 3.1 ± 0.3 4.3 ± 0.6 0.90 1.00 1.00 0.38 0.90 0.86 0.88 1.00 0.38 0.33
S100B+ 9.3 ± 1.8 8.0 ± 2.1 7.7 ± 0.9 8.5 ± 2.8 9.5 ± 1.8 0.78 0.66 0.96 1.00 1.00 1.00 0.66 0.96 0.52 0.90
Optic nerve SMI-32 0.7 ± 0.1 0.8 ± 0.1 1.3 ± 0.0 0.9 ± 0.1 1.0 ± 0.1 0.61 < 0.001 0.20 0.02 0.003 0.93 0.34 0.02 0.19 0.79
Cl. casp. 3+ 42.6 ± 5.1 43.1 ± 4.6 84.9 ± 8.6 45.0 ± 6.3 59.0 ± 9.2 1.00 0.004 1.00 0.53 0.003 1.00 0.51 0.004 0.10 0.63
Iba1+ 78.5 ± 6.0 83.7 ± 4.4 130.0 ± 9.3 72.4 ± 11.8 90.4 ± 10.6 1.00 0.003 1.00 0.87 0.009 0.90 0.98 0.001 0.03 0.61
Tmem119+ + Iba1+ 77.0 ± 6.1 80.7 ± 3.8 124.1 ± 9.2 70.2 ± 11.7 87.4 ± 10.1 1.00 0.006 0.98 0.91 0.01 0.91 0.98 0.002 0.04 0.63
Tmem119- + Iba1+ 1.5 ± 0.9 3.0 ± 1.5 6.3 ± 3.0 2.2 ± 1.0 3.0 ± 1.9 0.97 0.35 1.00 0.97 0.69 1.00 1.00 0.51 0.69 1.00
ED1+ + Iba1+ 65.4 ± 10.6 85.7 ± 11.8 163.3 ± 14.0 85.1 ± 7.0 84.5 ± 10.0 0.69 < 0.001 0.70 0.73 < 0.001 1.00 1.00 < 0.001 < 0.001 1.00
H&E 0.7 ± 0.2 1.0 ± 0.2 1.9 ± 0.2 0.8 ± 0.2 1.1 ± 0.2 0.83 0.003 0.98 0.66 0.04 0.99 1.00 0.01 0.08 0.92
GFAP+ 13.2 ± 1.2 13.7 ± 2.2 21.8 ± 2.2 18.8 ± 2.1 19.5 ± 2.0 1.00 0.04 0.30 0.20 0.06 0.38 0.26 0.82 0.92 1.00
S100B+ 9.6 ± 1.4 10.4 ± 1.7 8.3 ± 2.4 9.4 ± 1.6 7.3 ± 0.7 1.00 0.98 1.00 0.85 0.89 0.99 0.68 0.99 0.99 0.89