From: Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model
 | Cell type | Mean ± SEM | P-values | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  | Naïve | PBS | S100B | 13.5 mg/kg mino | 25 mg/kg mino | Naïve vs. PBS | Naïve vs. S100B | Naïve vs. 13.5 mg/kg mino | Naïve vs. 25 mg/kg mino | PBS vs. S100B | PBS vs. 13.5 mg/kg mino | PBS vs. 25 mg/kg mino | S100B vs. 13.5 mg/kg mino | S100B vs. 25 mg/kg mino | 13.5 vs. 25 mg/kg mino |
Retina | RBPMS+ | 276.8 ± 13.6 | 266.3 ± 19.0 | 161.1 ± 22.9 | 230.3 ± 28.5 | 236.0 ± 22.0 | 1.00 | 0.008 | 0.56 | 0.68 | 0.02 | 0.77 | 0.86 | 0.20 | 0.14 | 1.00 |
Cl. casp. 3+ + Brn3a+ | 2.6 ± 0.5 | 3.5 ± 1.7 | 9.5 ± 1.0 | 3.3 ± 0.8 | 6.2 ± 1.4 | 1.00 | 0.002 | 1.00 | 0.20 | 0.008 | 1.00 | 0.45 | 0.006 | 0.27 | 0.40 | |
Iba1+ | 10.1 ± 1.0 | 11.7 ± 0.5 | 19.0 ± 0.8 | 11.1 ± 0.8 | 11.5 ± 1.0 | 0.66 | < 0.001 | 0.90 | 0.74 | < 0.001 | 1.00 | 1.00 | < 0.001 | < 0.001 | 1.00 | |
Tmem119 + Iba1+ | 9.7 ± 0.9 | 11.3 ± 0.4 | 18.6 ± 0.7 | 10.9 ± 0.7 | 10.9 ± 0.9 | 0.57 | < 0.001 | 0.77 | 0.78 | < 0.001 | 1.00 | 1.00 | < 0.001 | < 0.001 | 1.00 | |
Tmem119- + Iba1+ | 3.0 ± 1.5 | 3.0 ± 1.5 | 3.0 ± 1.5 | 1.5 ± 0.9 | 1.5 ± 0.9 | 1.00 | 1.00 | 0.92 | 0.92 | 1.00 | 0.92 | 0.92 | 0.92 | 0.92 | 1.00 | |
ED1+ + Iba1+ | 1.3 ± 0.4 | 2.4 ± 0.9 | 5.2 ± 1.2 | 2.5 ± 1.4 | 2.4 ± 1.3 | 0.48 | < 0.001 | 0.40 | 0.48 | 0.002 | 1.00 | 1.00 | 0.002 | 0.002 | 1.00 | |
PKM+ | 14.8 ± 4.8 | 14.9 ± 5.3 | 7.5 ± 1.9 | 10.1 ± 3.9 | 9.8 ± 3.6 | 1.00 | 0.03 | 0.29 | 0.24 | 0.03 | 0.26 | 0.22 | 0.81 | 0.86 | 1.00 | |
GFAP+ | 3.1 ± 0.4 | 3.7 ± 0.4 | 3.1 ± 0.4 | 3.1 ± 0.3 | 4.3 ± 0.6 | 0.90 | 1.00 | 1.00 | 0.38 | 0.90 | 0.86 | 0.88 | 1.00 | 0.38 | 0.33 | |
S100B+ | 9.3 ± 1.8 | 8.0 ± 2.1 | 7.7 ± 0.9 | 8.5 ± 2.8 | 9.5 ± 1.8 | 0.78 | 0.66 | 0.96 | 1.00 | 1.00 | 1.00 | 0.66 | 0.96 | 0.52 | 0.90 | |
Optic nerve | SMI-32 | 0.7 ± 0.1 | 0.8 ± 0.1 | 1.3 ± 0.0 | 0.9 ± 0.1 | 1.0 ± 0.1 | 0.61 | < 0.001 | 0.20 | 0.02 | 0.003 | 0.93 | 0.34 | 0.02 | 0.19 | 0.79 |
Cl. casp. 3+ | 42.6 ± 5.1 | 43.1 ± 4.6 | 84.9 ± 8.6 | 45.0 ± 6.3 | 59.0 ± 9.2 | 1.00 | 0.004 | 1.00 | 0.53 | 0.003 | 1.00 | 0.51 | 0.004 | 0.10 | 0.63 | |
Iba1+ | 78.5 ± 6.0 | 83.7 ± 4.4 | 130.0 ± 9.3 | 72.4 ± 11.8 | 90.4 ± 10.6 | 1.00 | 0.003 | 1.00 | 0.87 | 0.009 | 0.90 | 0.98 | 0.001 | 0.03 | 0.61 | |
Tmem119+ + Iba1+ | 77.0 ± 6.1 | 80.7 ± 3.8 | 124.1 ± 9.2 | 70.2 ± 11.7 | 87.4 ± 10.1 | 1.00 | 0.006 | 0.98 | 0.91 | 0.01 | 0.91 | 0.98 | 0.002 | 0.04 | 0.63 | |
Tmem119- + Iba1+ | 1.5 ± 0.9 | 3.0 ± 1.5 | 6.3 ± 3.0 | 2.2 ± 1.0 | 3.0 ± 1.9 | 0.97 | 0.35 | 1.00 | 0.97 | 0.69 | 1.00 | 1.00 | 0.51 | 0.69 | 1.00 | |
ED1+ + Iba1+ | 65.4 ± 10.6 | 85.7 ± 11.8 | 163.3 ± 14.0 | 85.1 ± 7.0 | 84.5 ± 10.0 | 0.69 | < 0.001 | 0.70 | 0.73 | < 0.001 | 1.00 | 1.00 | < 0.001 | < 0.001 | 1.00 | |
H&E | 0.7 ± 0.2 | 1.0 ± 0.2 | 1.9 ± 0.2 | 0.8 ± 0.2 | 1.1 ± 0.2 | 0.83 | 0.003 | 0.98 | 0.66 | 0.04 | 0.99 | 1.00 | 0.01 | 0.08 | 0.92 | |
GFAP+ | 13.2 ± 1.2 | 13.7 ± 2.2 | 21.8 ± 2.2 | 18.8 ± 2.1 | 19.5 ± 2.0 | 1.00 | 0.04 | 0.30 | 0.20 | 0.06 | 0.38 | 0.26 | 0.82 | 0.92 | 1.00 | |
S100B+ | 9.6 ± 1.4 | 10.4 ± 1.7 | 8.3 ± 2.4 | 9.4 ± 1.6 | 7.3 ± 0.7 | 1.00 | 0.98 | 1.00 | 0.85 | 0.89 | 0.99 | 0.68 | 0.99 | 0.99 | 0.89 |