Fig. 5From: Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated miceSurgery up-regulates CXCR5 in mouse hippocampus. a Relative level of CXCR5 mRNA was measured by RT-qPCR on days 1, 3, and 7 after surgery. b Western blotting analysis and densitometry of CXCR5 protein levels. β-actin was used as an internal control (n = 8 per group). c Representative images of CXCR5 immunohistochemistry in the hippocampal CA1, CA3, and dentate gyrus. Positively stained cells are brown (red arrow). Magnification, × 5 for total hippocampus; × 20 for hippocampal CA1, CA3, and DG (n = 4 per group). *P < 0.05 vs. control. CXCR5 C-X-C motif chemokine receptor 5, DG dentate gyrus, RT-qPCR reverse transcription-quantitative polymerase chain reactionBack to article page