Fig. 1

Timelines for experimental protocols. Eight to 10-week-old C57BL/6 mice were used in various experimental protocols. Prior to CSN surgery, some experiments had organ/nerve excisions (b), and in others, blocking drugs were administered (c, d). In all instances with recovery, mice recovered in a heated cage (a–d, f, g). LPS was administered IP (200 μl) in all instances at 100 μg (a–d, f, except g) when a lethal dose of LPS was used (1 mg–200 μl). When stimulation was required (a–g), it was administered at 600 μA + 10 Hz, 400 μA + 10 Hz or 200 μA + 5 Hz and 0.1 ms in all instances. Stimulation occurred during unconsciousness (a–e) and consciousness (f, g). Experiments were typically conducted at least twice with an n number of 6–8