Fig. 5
CSN electrostimulation attenuates inflammation via glucocorticoids signalling in myeloid cells. C57BL/6 mice (a–c) or LysM-Cre:GRfl/fl mice (d) were obtained, aged 8–10 weeks, and CSN isolation and stimulation (600 μA, 10 Hz) were conducted. An LPS IP injection (100 μg) was administered in all animals; 90 min later, blood was collected for serum analysis by assay. a Corticosterone was measured in sham and CSN electrostimulated animals. b Impact of bilateral adrenal gland removal prior to CSN isolation and stimulation on LPS-induced serum TNF levels and serum corticosterone levels. c Impact of mifepristone (80 mg/kg) or vehicle (10% DMSO) IP administration prior to CSN isolation and stimulation on LPS-induced serum TNF levels. d LysM-Cre:GRfl/fl mice and their littermate controls, GRloxp/loxp, were obtained, and both received electrostimulation of the CSN. LPS-induced serum TNF levels were measured by ELISA. All individual traces, or points, represent one animal. The means are represented as ± SD