Fig. 7
Conscious CSN stimulation is protective against LPS-induced shock. Eight to 12-week-old wild-type and transgenic C57BL/6 mice underwent surgery 4 days prior to stimulation (a–c). a, b Stimulation (200 μA, 5 Hz, 2 × 2 min) was applied on freely moving mice, and LPS (5 mg/kg) was injected IP. All individual points represent one animal, and the means are expressed as ± SD. a Impact of CSN electrostimulation in conscious animals on LPS-induced serum TNF levels. b LysM-Cre:GRfl/fl mice and littermate controls underwent the same procedure as in a. c Wild-type C57BL/6 mice were implanted with an electrode on their CSN and stimulated (n = 16, 200 μA, 5 Hz, 5 min) or not (n = 13–16) twice a day for the next 3 days. A lethal dose of LPS (20 mg/kg) was administered to the mice (1 mg) IP. Animal survival was monitored