Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation

Fig. 2

Examination of brain myeloid populations in pre-symptomatic Tg-SwDI (“Pre-CAA”) versus symptomatic Tg-SwDI (“CAA”) reveals that the CD45highCD11b+cells are predominantly Tmem119+P2RY12+. a Pre-CAA brain (gated on live cells) myeloid populations show that nearly all CD11b+CD45int cells are double-positive Tmem119+P2RY12+ while no subpopulation of Tmem119+P2RY12+ cells resides within the CD11b+CD45high gate. b CAA brain myeloid populations show that nearly all CD11b+ CD45int cells are double-positive Tmem119+P2RY12+ and show a significant subpopulation of Tmem119+P2RY12+ cells within the CD11b+CD45high gate. c tSNE plots for the global examination of Live Tmem119+ pool in Pre-CAA brain. d tSNE plots for the global examination of Live Tmem119+ pool in the CAA brain. e Relative frequency of CD45int population as a percentage of Live Tmem119+ cells is significantly lower in CAA. f Relative frequency of CD45high population as a percentage of Live Tmem119+ cells is significantly higher in CAA. g Median fluorescence intensity (MFI) of CD45, as a measure of surface expression, in Live Tmem119+ cells is significantly higher in CAA. h Surface expression of Tmem119 in Live Tmem119+ cells is significantly lower in CAA. i Surface expression of P2RY12 in Live Tmem119+ cells is significantly lower in CAA. j Surface expression of MHCII in Live Tmem119+ cells is significantly higher in CAA. (n = 4/gp, unpaired student t test, ***p < 0.001, ****p < 0.0001)

Back to article page