Fig. 5

Ex vivo LPS stimulation of sorted MG (identified as CD45^intCD11b⁺ in naïve young brain) increases CD45 and decreases P2RY12 and Tmem119 surface expressions. Gating strategy used for sorting Live CD45^intCD11b⁺cells for 12-h ex vivo LPS stimulation (a). CD45 expression on sorted MG significantly increases after ex vivo LPS stimulation for 12 h (b). P2RY12 and Tmem119 expressions on sorted MG significantly decrease after ex vivo LPS stimulation for 12 h (c, d). (n = 10/gp, Wilcoxon matched-pairs signed-rank test (non-parametric t test), **p < 0.01)