Fig. 1

LPC-induced demyelination was ameliorated by Hv1 deficiency. a Illustration of injection positions (1.0 mm lateral, 0.6 mm anterior, 2.1 mm deep and 1.0 mm lateral, 1.1 mm anterior, 2.4 mm deep) and detection time points. b White matter lesions were detected by LFB staining at 5, 10, and 28 days post-injection (dpi) (scale bar, 200um). c Summarized data shows the severity of CC white matter lesions in histogram. White matter damage score: normal (grade 0). Disordered arrangement of nerve fibers (grade 1). Obvious cavitation formation (grade 2). Disappearance of myelin sheath fibers (grade 3). Two-way ANOVA with Dunnett’s post hoc test. ***P < 0.001, N ≥ 7 per group. d Representative images of MBP immunostaining in the CC of WT and Hv1^−/− mice. Lesion area outlined with a white dashed line (scale bar, 200 μm). e Quantification of the percentage of MBP loss area at three-time points. Each point of WT mice, N = 5-8 mice; Hv1^−/− mice, N = 6-8 mice. f Western blot analysis of MBP expression. g Quantification of the MBP intensity was measured and calculated as fold change over contralateral. N = 7-8 mice for each group. h Representative images of APC and Olig2 immunostaining in the CC of WT and Hv1^−/− mice (scale bar, 30 μm). i Quantification of the ratio of APC⁺/Olig2⁺. Each point of WT mice, N = 6-8 mice; Hv1^−/− mice, N = 7-8 mice. Data are shown as mean ± SD, *P < 0.05, **P < 0.01, two-way ANOVA with Dunnett’s post hoc test