Fig. 2

Microglial activation was attenuated by Hv1 deletion following LPC-induced demyelination. a Representative images of Iba-1 immunostaining in the CC of WT and Hv1^−/− mice (scale bar, 200 μm). b Quantification of the number of microglia per high-power field (HPF) in the CC. Each point of WT and Hv1^−/− mice, N = 5-7 mice. c Representative images of Iba-1 morphology and the corresponding 3D reconstructions (scale bars, magnified images, 20 μm; 3D reconstruction images, 5um) d Quantification analysis of the soma of microglia. Each point of WT and Hv1^−/− mice, N = 4-6 mice, 6-15 cells per mouse. e Representative images of Iba-1 and CD16/32 co-localization in the CC of WT and Hv1^−/− mice (scale bar, 50 μm; magnified images, 20 μm). f Quantification of the ratio of CD16/32⁺/Iba-1⁺. Each point of WT mice, N = 5-8 mice; Hv1^−/− mice, N = 5-8 mice. g Representative images of Iba-1 and CD206 co-localization in the CC of WT and Hv1^−/− mice (scale bar, 50 μm; magnified images, 20 μm). h Quantification of the ratio of CD206⁺/Iba-1⁺. Each point of WT mice, N = 6-7 mice; Hv1^−/− mice, N = 5-6 mice. Data are shown as mean ± SD, *P < 0.05, ***P<0.001, two-way ANOVA with Dunnett’s post hoc test