Table 1 Representative transfer EAE induction protocols in the literature
Publication | Genotype of recipient mice | Age of recipient mice (weeks) | Cell count (mio.) | T cell subset | Route | T-cell donor pre-immunization | Organ |
|---|---|---|---|---|---|---|---|
Williams et al. 2020 [48] | C57BL/6J | 8-9 | 10 | Th1 | Retro-orbital i.v. | 50 μg MOG (aa35-55) in IFA with 500 μg/ml M. tuberculosis | Spleen |
Stromnes et al. 2008 [4] | Sublet. irradiated C3HeB/Fej mice | Unknown | 20 | Th1 Th17 | i.p. | rMOG-immunized mice for 3 days with rMOG (25 μg/ml) or MOG peptides | Spleen |
Domingues et al. 2010 [19] | Rag2−/− | Unknown | 5-10 | Th1 Th17 both | Tail vein i.v. | No, but 2d2 or OSE genotype | Spleen |
Kroenke et al. 2010 [49] | C57BL/6J | 8-12 | 5-6 | Th1 | i.p. | s.c. 100 μg MOG (aa35–55) in complete FA | LN |
Rothhammer et al. 2011 [17] | Rag1−/− | Unknown | 2 of cytokine positive cells | Th1 Th17 | i.v. | No, but 2d2 | Spleen, LN |
Jäger et al. 2009 [18] | C57BL/6J | 7-10 | 3-5 | Th1 Th17 | i.v. | No, but 2d2 | Spleen, LN |
Our protocol | Rag2−/− | 8-12 | 5-10 | Th1 Th17 | Tail vein i.v. | No, but 2d2 | Spleen, LN |