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Table 1 Representative transfer EAE induction protocols in the literature

From: Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy

Publication Genotype of recipient mice Age of recipient mice (weeks) Cell count (mio.) T cell subset Route T-cell donor pre-immunization Organ
Williams et al. 2020 [48] C57BL/6J 8-9 10 Th1 Retro-orbital i.v. 50 μg MOG (aa35-55) in IFA with 500 μg/ml M. tuberculosis Spleen
Stromnes et al. 2008 [4] Sublet. irradiated
C3HeB/Fej mice
Unknown 20 Th1
Th17
i.p. rMOG-immunized mice for 3 days with rMOG (25 μg/ml) or MOG peptides Spleen
Domingues et al. 2010 [19] Rag2−/− Unknown 5-10 Th1
Th17 both
Tail vein i.v. No, but 2d2 or OSE genotype Spleen
Kroenke et al. 2010 [49] C57BL/6J 8-12 5-6 Th1 i.p. s.c. 100 μg MOG (aa35–55) in complete FA LN
Rothhammer et al. 2011 [17] Rag1−/− Unknown 2 of cytokine positive cells Th1
Th17
i.v. No, but 2d2 Spleen, LN
Jäger et al. 2009 [18] C57BL/6J 7-10 3-5 Th1
Th17
i.v. No, but 2d2 Spleen, LN
Our protocol Rag2−/− 8-12 5-10 Th1
Th17
Tail vein i.v. No, but 2d2 Spleen, LN
  1. Th T helper cell. i.v. intravenous, MOG myelin oligodendrocyte glycoprotein, IFA incomplete Freund’s adjuvant, i.p. intraperitoneal, FA Freund’s adjuvant, LN lymph node