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Table 2 Differential cytokine applications for Th1 and Th17 induction

From: Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy

Publication

APC (106)

MOG (aa35-55)

Anti-CD28

Anti-CD3

IL-12

IL-2

IL-18

Anti-IL-4

rmIFNγ

IL-23

hTGF-β

IL-6

Anti-IFNγ

Restimulation in vitro

Williams et al. 2020 [48]

5

10 μg/ml

/

/

10 U/ml (Th1)

10 U/ml (Th1)

/

/

/

/

/

/

/

Yes

Stromnes et al. 2008 [4]

/

/

/

/

10 ng/ml (Th1)

/

/

/

/

10 ng/ml (Th17)

/

/

/

No

Domingues et al. 2010 [19]

/

20 μg/ml (MOG aa1-125)

/

/

10 ng/ml (Th1)

10 ng/ml at day 3 (Th1)

25 ng/ml (Th1)

10 μg/ml

(Th1)

(Th17)

/

10 ng/ml

(Th17)

5 ng/ml (Th17)

20 ng/ml (Th17)

/

Yes

Kroenke et al. 2010 [49]

 

4 days with MOG

/

/

5 ng/ml (Th1)

/

/

10 μg/ml (Th1)

2 ng/ml (Th1)

/

/

/

/

No

Rothhammer et al. 2011 [17]

Irradiated 1:5 ratio

20 μg/ml

(Th1)

(Th17)

2 μg/ml plate-bound

(Th1)

(Th17)

4 μg/ml plate-bound

(Th1)

(Th17)

10 ng/ml (Th1)

/

/

10 μg/ml

(Th1)

(Th17)

/

25 ng/ml (Th17)

2 ng/ml (Th17)

20 ng/ml (Th17)

/

Yes

Jäger et al. 2009 [18]

Irradiated 1:5 ratio

20 μg/ml

/

2.5 μg/ml (Th1)

(Th17)

10 ng/ml (Th1)

/

/

20 μg/ml

(Th1)

(Th17)

/

/

3 ng/ml

(Th17)

30 ng/ml (Th17)

20 μg/ml (Th17)

Yes

Our protocol

Irradiated 1:5 (Th1) ratio

1:10 (Th17)

/

/

2 μg/ml

(Th1)

(Th17)

5 ng/ml (Th1)

/

25 ng/ml (Th1)

10 μg/ml

(Th1)

(Th17)

/

20 ng/ml (Th17)

3 ng/ml (Th17)

20 ng/ml (Th17)

10 μg/ml (Th17)

Yes

  1. MOG myelin oligodendrocyte glycoprotein