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Table 2 Differential cytokine applications for Th1 and Th17 induction

From: Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy

Publication APC (106) MOG (aa35-55) Anti-CD28 Anti-CD3 IL-12 IL-2 IL-18 Anti-IL-4 rmIFNγ IL-23 hTGF-β IL-6 Anti-IFNγ Restimulation in vitro
Williams et al. 2020 [48] 5 10 μg/ml / / 10 U/ml (Th1) 10 U/ml (Th1) / / / / / / / Yes
Stromnes et al. 2008 [4] / / / / 10 ng/ml (Th1) / / / / 10 ng/ml (Th17) / / / No
Domingues et al. 2010 [19] / 20 μg/ml (MOG aa1-125) / / 10 ng/ml (Th1) 10 ng/ml at day 3 (Th1) 25 ng/ml (Th1) 10 μg/ml
(Th1)
(Th17)
/ 10 ng/ml
(Th17)
5 ng/ml (Th17) 20 ng/ml (Th17) / Yes
Kroenke et al. 2010 [49]   4 days with MOG / / 5 ng/ml (Th1) / / 10 μg/ml (Th1) 2 ng/ml (Th1) / / / / No
Rothhammer et al. 2011 [17] Irradiated 1:5 ratio 20 μg/ml
(Th1)
(Th17)
2 μg/ml plate-bound
(Th1)
(Th17)
4 μg/ml plate-bound
(Th1)
(Th17)
10 ng/ml (Th1) / / 10 μg/ml
(Th1)
(Th17)
/ 25 ng/ml (Th17) 2 ng/ml (Th17) 20 ng/ml (Th17) / Yes
Jäger et al. 2009 [18] Irradiated 1:5 ratio 20 μg/ml / 2.5 μg/ml (Th1)
(Th17)
10 ng/ml (Th1) / / 20 μg/ml
(Th1)
(Th17)
/ / 3 ng/ml
(Th17)
30 ng/ml (Th17) 20 μg/ml (Th17) Yes
Our protocol Irradiated 1:5 (Th1) ratio
1:10 (Th17)
/ / 2 μg/ml
(Th1)
(Th17)
5 ng/ml (Th1) / 25 ng/ml (Th1) 10 μg/ml
(Th1)
(Th17)
/ 20 ng/ml (Th17) 3 ng/ml (Th17) 20 ng/ml (Th17) 10 μg/ml (Th17) Yes
  1. MOG myelin oligodendrocyte glycoprotein