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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: K284-6111 alleviates memory impairment and neuroinflammation in Tg2576 mice by inhibition of Chitinase-3-like 1 regulating ERK-dependent PTX3 pathway

Fig. 5

Inhibitory effect of K284-6111 on ERK and NF-κB pathway. a Level of p-IκBα, IκBα, p-p38, 38, p-ERK 1/2, ERK 1/2, p-JNK, and JNK were detected by Western blot in the Tg2576 mice brain. BV-2 cells were treated with Aβ (5 μM) and K284-6111 (0.5, 1, and 2 μM). b Level of p-IκBα, IκBα, p-p38, 38, p-ERK 1/2, ERK 1/2, p-JNK, and JNK were detected by Western blot. BV-2 cells were treated with Aβ (5 μM), U0126 (20 μM), SP600125 (20 μM), SB203580 (10 μM), and Bay 11-7082 (5 μM). (C) Level of p-ERK 1/2, ERK 1/2, p-IκBα, and IκBα were detected by Western blot. BV-2 cells were treated with Aβ (5 μM), K284-6111 (2 μM), and U0126 (20 μM). (D) Expression of iNOS, COX-2, and IBA-1 was detected by Western blot. (e) The mRNA expression level of pro-inflammatory cytokines (Tnf, Il1b, and Il6) and M1 microglia phenotype marker (Cd86) in BV-2 cells were assessed by qRT-PCR

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