Fig. 7

Effect of K284-6111 on PTX3-involved neuroinflammation. a Gene network analysis associated with CHI3L1 was carried out using the web-based analysis tool. BV-2 cells were treated with Aβ (5 μM) and K284-6111 (2 μM). (B) The mRNA expression level of Chi3l1 and Ptx3 in BV-2 cells was assessed by qRT-PCR. Expression of PTX3 was detected by Western blot (c) in the Tg2576 mice brain and (d) in the BV-2 cells. BV-2 cells were transfected with PTX3 siRNA (20 nM). After 24 hr, cells were treated with Aβ (5 μM). e The mRNA expression level of pro-inflammatory cytokines (Tnf, Il1b, and Il6) and M1 microglia phenotype marker (Cd86) in BV-2 cells were assessed by qRT-PCR. f Expression of iNOS and COX-2 was detected by Western blot. (G) Level of p-ERK 1/2, ERK 1/2, p-IκBα, and IκBα were detected by Western blot. BV-2 cells were treated with Aβ (5 μM), K284-6111 (2 μM), U0126 (20 μM), and Bay 11-7082 (5 μM). (H) Expression of PTX3 were detected by Western blot. BV-2 cells were transfected with CHI3L1 plasmid vector. After 24 hr, cells were treated with Aβ (5 μM), K284-6111 (2 μM), U0126 (20 μM), and Bay 11-7082 (5 μM). (I) Expression of PTX3 were detected by Western blot.