Fig. 6
From: CCN3 is dynamically regulated by treatment and disease state in multiple sclerosis
CCN3 immunostaining in normal and MS brain tissue. CCN3 is present in blood vessels, choroid epithelia and neuron-, and macroglia-like cells of the CNS (black arrows, a–d). Co-labelled sections of MS grey matter with antibodies to the mature neuronal protein HuC/D (green) and anti-CCN3 (red) demonstrates dual-labelling of CCN3 and HuC/D+ neurons (e). Detection of CCN3 transcripts by in situ hybridisation in MS grey matter with a distinct neuronal morphology (f) and respective negative control (f, inset). Area of CCN3 immunoreactivity in MS brain tissue (n = 17 cases) (g). GM, normal-appearing grey matter; GM Lesion, demyelinated GM, GM remy, remyelinated GM; WM, normal-appearing white matter; WM Lesion, demyelinated WM; WM remy, remyelinated WM