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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Crosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model

Fig. 2

Verification of microglia activation via morphological and protein expression analyses. a Immunofluorescence staining of Alexa488Phalloidin+ actin (green) showing HMC3 microglia morphology in MG single (elongated quiescent) or GBM-MG co-culture (ameboid, activated). Also, Hoechst 33342+ nuclei stained blue. b Western blot staining CD68 and β-actin in triplicate. MG single on the left and MG co-culture on the right. c CD68 protein expression levels (n = 3) in microglia increased as a result of GBM co-culture. *p < 0.05

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Journal of Neuroinflammation

ISSN: 1742-2094