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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice

Fig. 1

Oral Pg induced dopaminergic neuronal degeneration in the SNpc of mutant LRRK2 mice. a Representative images of immunofluorescence-stained coronal brain sections and quantification of TH number (a marker of dopaminergic neuron ) from the F + Pg and R1441G + Pg groups compared to F + C and R1441G + C mice. n = 4–5, *P < 0.05 (scale bar = 200 μm). Two-way ANOVA and Tukey’s test were used for analysis. b Representative images of TH+ dopaminergic neurons (green) and cleaved caspase-3 (red) (white arrows indicate co-localized cells) immunofluorescent staining from the SN in brain sections obtained from F + C, F + Pg, R1441G + Pg, and R1441G + C mice (scale bar = 50 μm). c Representative images of western blots of cleaved caspase-3 protein levels and quantitative analysis, which were done with the SN obtained from F + Pg and 1441 + Pg mice compared to F + C and 1441 + C mice. n = 4, **P < 0.01. Two-way ANOVA and Tukey’s test were used for analysis. d Representative images of immunofluorescence double staining with dendric marker MAP2 and comparison of dendric density from F + Pg, 1441 + Pg, F + C, and 1441 + C mice. n = 4–5, ***P < 0.001 (scale bar = 100 μm). Two-way ANOVA and Tukey’s test were used for analysis. e Representative images of western blots and quantitative analyses of MAP2 protein levels. Analyses of protein levels of MAP2 were done on the SN obtained from F + Pg and 1441 + Pg mice compared to F + C and 1441 + C mice. n = 4, **P < 0.01. Two-way ANOVA and Tukey’s test were used for analysis

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