Fig. 3

Oral Pg increased LRRK2 activation in the SN of R1441G mice. a Representative images of western blots of LRRK2 and LRRK⁹³⁵ protein levels and b quantitative analysis, which were done on the SN obtained from F + Pg and 1441 + Pg mice compared to F + C and 1441 + C mice. n = 4, ***P < 0.001,*P < 0.05. Two-way ANOVA and Tukey’s test were used for analysis. c Representative images of TH+ dopaminergic neuron by immunofluorescent double staining with LRRK2 (white arrows indicate co-localized cells) (scale bar = 50 μm). d Representative images of Iba1+ microglia by immunofluorescent double staining with LRRK2 (white arrows indicate the cells co-localized with LRRK2 and Iba1) (scale bar = 100 μm)