Fig. 5
Pg treatment increased the accumulation of α-synuclein in neurons of the colon and caused activation of LRRK2. a Representative images of α-synuclein (red) (white arrows) immunofluorescent staining from the colon obtained from F + C, F + Pg, 1441 + Pg, and 1441 + C mice (scale bar = 100 μm). Representative images of western blots of b LRRK2 and c LRRK935 protein levels, and quantitative analysis, which were done with the colon tissue obtained from the F + Pg and 1441 + Pg groups and compared with F + C and 1441 + C mice. n = 4, ****P < 0.0001,*P < 0.05. Two-way ANOVA and Tukey’s test were used for analysis. Comparison of relative d IL-1β, e TNF-α, and f Zo-1 gene expression levels in the colon from F + C, F + Pg, 1441 + Pg, and 1441 + C mice. n = 6–8, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-way ANOVA and Tukey’s test were used for analysis