Fig. 4
Expression of Il1b and Il1r genes across retinal cell clusters. a UMAP plot of droplet-based single-cell RNA sequencing (scRNA-seq) data obtained using 10× Genomics technology and representing retinal cell type from adult mouse retina (n = 4, 10–16 weeks). The plot shows a two-dimensional representation of global gene expression relationship among 33942 cells clustered into 12 retinal cell types (top panel). The expression levels of Il1r1 and Il1b are represented as dot plots across all the 12 cell types; larger dots indicate broader expression within the cluster; deeper red denotes a higher expression level (bottom panel). b UMAP plot of droplet-based scRNA-seq data obtained using 10× Genomics technology showing different clusters (top panel). UMAP plot of droplet-based single-cell RNA sequencing (scRNA-seq) data obtained using 10× Genomics technology and representing retinal cell types from control and light-damaged (LD) adult mouse retina. scRNA-seq data were generated on fluorescence-assisted cell sorting (FACS)-sorted live Cx3cr1YFP+ cells from pooled neuroretinas of normal (n = 5) and LD (n = 8) mice. MG: microglia; pv MFs: perivascular macrophages; mo MFs: monocyte-derived macrophages. The plot shows a two-dimensional representation of global gene expression relationship among 10582 cells clustered into 3 retinal cell types (bottom panel). c Bar plot representation of Cx3cr1YFP+ cell proportions in different conditions. d The expression levels of Il1r1 and Il1b are represented as dot plots across all the 3 immune cell types; larger dots indicate broader expression within the cluster; deeper red denotes a higher expression level. Representative confocal images showing co-immunoreactivity of IL-1R1 (green) with e GFAP+ cells (red) in the ganglion cell layer and f lectin (red). n = 4 per group. Scale bar 20 μm. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer